乙炔抑制法对农田土壤反硝化潜势不确定性的量化

曹文超; 王娅静; 李艳青; 潘好芹; 赵飞; 肖万里; 王翠翠; 王敬国; 宋贺

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中国环境科学 ›› 2024, Vol. 44 ›› Issue (10) : 5733-5742.

土壤污染与控制

乙炔抑制法对农田土壤反硝化潜势不确定性的量化

曹文超^1,2, 王娅静³, 李艳青¹, 潘好芹¹, 赵飞¹, 肖万里¹, 王翠翠¹, 王敬国⁴, 宋贺⁵

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Quantifying the uncertainty of farmland soil actual denitrification potential as determined by the acetylene inhibition method

CAO Wen-chao^1,2, WANG Ya-jing³, LI Yan-qing¹, PAN Hao-qin¹, ZHAO Fei¹, XIAO Wan-Li¹, WANG Cui-cui¹, WANG Jing-guo⁴, SONG He⁵

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摘要

通过利用Robot培养系统进行厌氧泥浆培养试验,旨在明确乙炔抑制法在定量设施菜田与粮田土壤实际的反硝化潜势时是否均存在低估现象.以寿光地区设施菜田土壤与相邻粮田土壤为研究对象,分别设置了添加10%乙炔和未添加10%乙炔处理.结果表明,厌氧泥浆培养3h后,设施菜田与粮田土壤在10%乙炔处理中的N₂O累积浓度均显著高于未添加乙炔处理,而N₂累积浓度呈现相反趋势;在10%乙炔下,设施菜田和粮田土壤在厌氧培养63h内分别有7.92%和34.2%的N₂O被还原为N₂;添加10%乙炔设施菜田与粮田土壤实际的反硝化潜势均显著增加,但并未显著影响土壤的呼吸作用;由乙炔抑制法对土壤实际的反硝化潜势计算的偏差(AIT-bias)来看,乙炔抑制法高估设施菜田土壤实际的反硝化潜势比例为22.0%,而对粮田土壤低估了11.4%.综合来看,在设施菜田与粮田土壤中,乙炔抑制法均未完全抑制N₂O还原为N₂;乙炔抑制法对土壤实际反硝化潜势的不确定性受种植系统的深刻影响,这可能与不同种植系统下土壤养分、土壤类型等因素存在差异有关,需要进一步评估该方法在不同土壤条件下的准确性.

Abstract

This study employed an anaerobic slurry incubation test to evaluate whether the acetylene inhibition method may have led to an underestimation of the denitrification potential in both greenhouse vegetable and grain field soils, using a robotized sampling and analysing system. Soils from greenhouse vegetable fields and adjacent grain fields in the Shouguang region were sampled. The treatments with and without the addition of 10% acetylene were set up respectively. The results showed that the accumulated N₂O concentration in the 10% acetylene treatment was significantly higher than those without acetylene after 3hours of anaerobic slurry incubation, while N₂ accumulation showed the opposite trend. Moreover, in the presence of acetylene, 7.92% and 34.2% of N₂O were reduced to N₂ in greenhouse vegetable and grain field soils, respectively, within 63 hours of anaerobic incubation. The actual denitrification potential significantly increased with the addition of 10% acetylene in the greenhouse vegetable and grain field soils, but the respiration of the soils was not significantly affected. Specifically, the acetylene inhibition method overestimated the actual denitrification potential in greenhouse vegetable soil by 22.0%, and underestimated it in grain field soil by 11.4%, as determined by the bias calculated from the acetylene inhibition method (AIT-bias). Overall, the acetylene inhibition method was found to be insufficient in completely suppressing N₂O reduction to N₂ in greenhouse vegetable and grain field soils. The uncertainty of acetylene inhibition method on the actual denitrification potential of soil is profoundly influenced by the cropping system, which may be related to variations in soil nutrients, types and other factors. Further research is necessary to assess the reliability of this method across different soil conditions.

导出引用

曹文超, 王娅静, 李艳青, 潘好芹, 赵飞, 肖万里, 王翠翠, 王敬国, 宋贺. 乙炔抑制法对农田土壤反硝化潜势不确定性的量化[J]. 中国环境科学. 2024, 44(10): 5733-5742

CAO Wen-chao, WANG Ya-jing, LI Yan-qing, PAN Hao-qin, ZHAO Fei, XIAO Wan-Li, WANG Cui-cui, WANG Jing-guo, SONG He. Quantifying the uncertainty of farmland soil actual denitrification potential as determined by the acetylene inhibition method[J]. China Environmental Science. 2024, 44(10): 5733-5742

中图分类号： X142

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