Abstract：Pollution sources of the typical rural pond-drinking water were studied in a country of Guangdong using molecular typing methods such as pulsed-field gel electrophoresis (PFGE), ERIC-PCR and BOX-PCR. Escherichia coli was the indicating microorganism in microbial source tracking (MST). The fingerprints of E. coli from different samples were analyzed using Quantity One. It was showed that the superficial layer well had the same genotyping E. coli with the pond water beside it. Therefore, they might have the same pollution carrier. Or the pond resulted in the pollution of superficial layer well. The ability in discriminating E. coli strains was different, such as PFGE>BOX-PCR>ERIC-PCR. BOX-PCR was the most suitable molecular typing method for microbial source tracking. In view of limitation of the culture-dependent methods, we should pay more attention to the combination with culture-independent methods in the future MST.
冯广达, 邓名荣, 郭俊, 朱红惠, 羊宋贞, 梁浩亮, 朱昌雄. 广东某农村塘坝饮用水污染的微生物溯源[J]. 中国环境科学, 2011, 31(1): 96-104.
FENG Guang-Da, DENG Ming-Rong, GUO Jun, ZHU Hong-Hui, YANG Song-Zhen, LIANG Hao-Liang, ZHU Chang-Xiong. Microbial source tracking of the pollution in pond-drinking water in a country of Guangdong. CHINA ENVIRONMENTAL SCIENCECE, 2011, 31(1): 96-104.
