Abstract:Change of microbe community in tower earthworm ecology-filter was studied in this research with PCR-DGGE technology (polymerase chain reaction-denaturing gradient gel electrophoresis). These results showed that: the change of microbe community was obvious and linked with hydraulic resident time、pollutation burden and the volume of filters. Meanwhile, Shannon-Wiener Index of biodiversity increased from 0.25 to 1.27 in an all-round way, including 0.29~1.05 in the first filter, 0.75~0.97 in the second filter and 0.87~0.25~1.27 caused by some glucose added into influent in the last filter. Under the same reason, the activity and light of denitrifying bacteria were intensified on bluestone and gravel layers. After analysis of cloning sequencing and phylogenetic tree, most of the inferred bacteria were Sphingomonas, Pseudomonas, Gram-negative bacteria and uncultured Bacillus and changed evidently in different filters.
郭飞宏, 郑正, 张继彪. PCR-DGGE技术分析塔式蚯蚓生态滤池微生物群落结构[J]. 中国环境科学, 2011, 31(4): 597-602.
GUO Fei-Hong, ZHENG Zheng, ZHANG Ji-Biao. Research on microbe community in tower earthworm ecology-filter by PCR-DGGE. CHINA ENVIRONMENTAL SCIENCECE, 2011, 31(4): 597-602.