Abstract:Effect of subchronic perfluorooctane sulfonate (PFOS) exposure on free calcium level ([Ca2+]i) in hippocampus in rats and the mechanism of the neurotoxicity induced by PFOS was studied. The adult male Wistar rats were treated with oral PFOS exposure at dosage of 2, 8, 32, 128mg/kg, respectively. After 60 days’ exposure, the rats were sacrificed, and then single-cell suspension of hippocampus was prepared. [Ca2+]i was determined with Fura-2/AM fluorescence probe. The brain and serum were concentrated by liquid–phase extraction and then the concentration of PFOS was determined using HPLC/MS. The cortex, hippocampus and cerebellum were collected for evaluation of histopathology change. PFOS concentration in serum and brain in PFOS treated groups was significantly higher than that in the control group (P<0.01). There was a significant positive correlation between [Ca2+]i and PFOS concentration in serum and brain. No obvious change was observed in the morphology of the brain in rats exposed to PFOS.
刘晓晖, 刘冰, 金一和. 亚慢性全氟辛烷磺酸染毒对大鼠海马细胞钙稳态的影响[J]. 中国环境科学, 2010, 30(1): 110-115.
LIU Xiao-Hui, LIU Bing, JIN Yi-He. Effect of perfluorooctane sulfonate on intracellular free calcium level in rats’ hippocampus. CHINA ENVIRONMENTAL SCIENCECE, 2010, 30(1): 110-115.