Abstract：To enhance the degradation efficiency of pyrene co-metabolism by indigenous white-rot fungus Pseudotrametes gibbosa from northeast forest area in China, orthogonal experiments were designed to get the optimum conditions for pyrene co-metabolism, and the effects of different pyrene concentrations on the degradation efficiency of pyrene co-metabolism were also investigated. The optimum conditions were as following: wheat bran with the concentration of 20g/L, three agar plugs (10mm diameter) and 50mL culture in 150mL flask without ABTS. The maximum laccase (Lac) production and the degradation efficiency of pyrene reached 53.41U/mL and 88.8% respectively under the optimum conditions. Lac secretion was enhanced when pyrene concentration was lower than 10 mg/L, but it was inhibited at pyrene concentration above 10 mg/L. At the same time, the degradation efficiency of pyrene was ranged from 22.24%~93.54% if the initial concentration of pyrene was lower than 90 mg/L.
