Abstract:E. coli and Shigella spp. were detected and differentiated using surface-enhanced Raman scattering (SERS) by preparing bacteria samples with silver colloids on a silicon surface. Results show that Raman signals of E. coli and Shigella spp. were negligible in the absence of silver nanoparticles, but observable and distinguishable spectra were collected while in the presence of silver nanoparticles. Thus, E. coli and Shigella spp. could easily be discriminated based on their different Raman spectra. The method in this work exhibited high reproducibility and showed great promise for the detection of E. coli and Shigella spp. in environmental samples.
吕璞, 龚继来, 王喜洋, 曾光明, 崔卉, 胡家文. 表面增强拉曼散射技术鉴别大肠杆菌和志贺氏菌的研究[J]. 中国环境科学, 2011, 31(9): 1523-1527.
吕Pu , GONG Ji-Lai, WANG Xi-Yang, ZENG Guang-Ming, CUI Hui, HU Jia-Wen. Detection and discrimination of Escherichia coli and Shigella spp. using surface-enhanced Raman spectroscopy. CHINA ENVIRONMENTAL SCIENCECE, 2011, 31(9): 1523-1527.