Abstract:The nitrification from ammonia to nitrate is a rate-limiting step mediated by ammonia-oxidizing bacteria (AOB). The abundances and diversity of AOB in the soil of reed wetland of Liaohe Estuary were investigated by the most probable number (MPN) method and the denaturing gradient gel electrophoresis (DGGE) technique,and the relationship between AOB abundances and environmental factors, such as soluble salt, was further explored. The results indicated that the temporal and spatial distribution of the AOB diversity was different, and the bacterial community on the different sites was more similar in same month. The quantity of AOB and the concentration of soluble salt presented notable negative correlation(P<0.05), while the quantity of AOB and soil total nitrogen and organ matter presented a significant positive correlation (P<0.01). It concluded that the soluble salt, soil total nitrogen and orgaic matter had an important influence on the quantity of AOB in Liaohe Estuary wetland.
白洁, 董晓, 赵阳国. 辽河口芦苇湿地土壤氨氧化菌的时空变化[J]. 中国环境科学, 2011, 31(11): 1870-1874.
BAI Jie, DONG Xiao, ZHAO Yang-Guo. Characteristics of temporal and spatial distribution of ammonia-oxidizing bacteria in Liaohe Estuary wetland soil. CHINA ENVIRONMENTAL SCIENCECE, 2011, 31(11): 1870-1874.