Abstract:Communities of AOB in activated sludges collected from 4 wastewater treatment systems in 2 plants in Beijing were examined using specific PCR followed by terminal restriction fragment length polymorphism (T-RFLP), cloning, and sequencing of amoA genes. Communities of bacteria were also investigated by using T-RFLP based 16S rRNA genes. The T-RFLP fingerprints analysis showed that the dominant terminal restriction fragments (T-RFs) of AOB were 291, 354 bp, and the dominant T-RFs of bacteria were 115, 117, 166, 455, 465, 468, 471, 482, 800 and 893 bp etc. in all these 4 systems. The T-RFLP profiles analysis may also indicate the AOB and bacteria structures were slightly affected by the treatment process. Phylogenetic analysis of cloned amoA genes clearly showed that the dominant AOB in these 4 systems were members of Nitrosomonas europaea cluster and Nitrosomonas oligotropha cluster.
王晓慧, 文湘华, 杨宁宁, 许美兰, 丁, 鵾. 污水处理工艺对氨氧化菌及细菌群落的影响[J]. 中国环境科学, 2009, 29(6): 622-628.
WANG Xiao-Hui, WEN Xiang-Hua, YANG Ning-Ning, XU Mei-Lan, DING , KUN . Impacts of wastewater treatment process on AOB and bacteria communities. . CHINA ENVIRONMENTAL SCIENCECE, 2009, 29(6): 622-628.