Abstract:Two bacterial strains, H2 and H4, were isolated from marine sediment and identified as Bacillus pumilus H2 and Bacillus licheniformis H4, respectively, based on their physiological properties and the analysis of 16S rRNA gene sequence. Both strains showed significantly inhibitory effects against the growth of Vibrio natriegens by antimicrobial activity assay. However, only strain H2 could survive in the intestinal conditions as revealed by the simulated gastro intestinal tract (GIT) test, keeping cell number stably at 1010 CFU/mL. B. pumilus H2 was also benefit to the survival of juvenile shrimp (Litopenaeus vannamei) as revealed by the flask bottle test (20 shrimp fries for every bottle as a group). Probiotic effect of B. pumilus H2 to juvenile shrimp was carried out in aquaculture tanks with 100L each with 50 shrimps. Juvenile shrimp were exposed to B. pumilus H2 at 0 (as control), 103 and 104 CFU/mL for 14d before a challenge with Vibrio natriegens at 104 CFU/mL for 1 d infection. The final mortality of the shrimp group treated with 104 CFU/mL B. pumilus H2 was only 12.5%, much lower than the group treated with 103 CFU/mL B. pumilus H2 (28.3%) and the control group (30.8%, P<0.05); and the average weight and length of the shrimp group treated with 104 CFU/mL B. pumilus H2 were also a bit higher than those of the control group.
傅松哲, 宋奔奔, 刘鹰, 刘志培. 弧菌拮抗菌的筛选及其对凡纳滨对虾的抑菌防病作用[J]. 中国环境科学, 2009, 29(8): 867-872.
FU Song-Zhe, SONG Ben-Ben, LIU Ying, LIU Zhi-Pei. Screening of probiotic strains and their protection against vibriosis in juvenile shrimp (Litopenaeus vannamei).. CHINA ENVIRONMENTAL SCIENCECE, 2009, 29(8): 867-872.