Abstract:The formaldehyde-degradation characteristics of three strains of formaldehyde-degrading fungi (Trichoderma viride H1, Penicillium javanicum H2, Aspergillus flavus H4) in different carbon sources media was studied. Four kinds of complex carbon sources (0.1% formaldehyde with 10% sucrose, 10% maltose, 10% starch, and 10% glucose, respectively) could be used by the three strains of formaldehyde-degrading fungi and the formaldehyde degradation rate were arrived at 99.3%, 70.1% and 99.7%, respectively. The results of using formaldehyde as the sole carbon source showed that the formaldehyde degradation rate were 88.4%, 64.1% and 63.0%, respectively. The growth of the mycelium of H1 was obtained.