Abstract：A Tn7-based transposon was randomly inserted into genomic DNA of Pantoea agglomerans BH18, isolated from mangrove sludge. Mutants were screened by Kanr and amplification of the inserted sequences. At the initial pH 4.0, an aciduric and highly effective hydrogen producing mutant TB220 was screened using hydrogen production as screening index. The aciduric mutant TB220 was tested to have steady heredity and hydrogen-producing capability in several passages. The mutant TB220 was able to produce hydrogen over a wide rang of initial pH from 3.5 to 7.0, with an optimum initial pH of 6.0, and hydrogen production was (2.39 ± 0.08)mol H2/mol glucose. Under the marine conditions with the initial pH of 4.0 and glucose concentration of 10 g/L, hydrogen production of the mutant TB220 was (0.47 ± 0.02) mol H2/mol glucose, increasing by 70% compared with wild type. This indicated that the mutant showed high acid resistance capability.
刘洪艳, 朱大玲, 王文磊. 一株耐酸产氢突变株Pantoea agglomerans的筛选与产氢特性[J]. 中国环境科学, 2012, 32(1): 125-129.
LIU Hong-Yan, ZHU Da-Ling, WANG Wen-Lei. Screening of an aciduric hydrogen producing mutant Pantoea agglomerans and characterization of hydrogen production. CHINA ENVIRONMENTAL SCIENCECE, 2012, 32(1): 125-129.
