Abstract：The five single factors for cellulose biodegradation ability of cellulase producing strains were optimized by orthogonal experiments using mixed carbon source under 30°C, and were used in wastewater treatment system with anaerobic and aerobic processes. Five single culture factors promoted cellulase activity and cellulose degradation-rate in varying degrees. Moreover, the optimal culture conditions of anaerobe obtained through orthogonal experiments were not in accordance with that of aerobe. In the wastewater treatment system, the optimal synthetic condition was MgSO4 of 30mg/L, CoCl2 of 20mg/L , the ratio of CNP of 400:5:1, NH4Cl (of 28.7mg/L) as nitrogen source and pH7.0 . The cellulase activities (CA), enzymatic stabilities (ES), and cellulose degradation-rates (CDR) of anaerobe and aerobe were as follows: CA: 4801U/L, 4794U/L; ES: 91.0%, 95.5%; CDR: 31.9%, 28.4% .
