Abstract：The coal sample taken from Yinchuan, coal-fired PM2.5 was sampled by fixed source dilution channel and PM2.5 suspension was extracted by ultrasonic water-bath method. Human umbilical vein endothelial cells were treated with PM2.5 suspension at various concentrations and exposed for different times (6h, 12h, and 24h). The MTS assay was used to measure the effect of PM2.5 on cell proliferation of EA. hy926. Coal-fired PM2.5 suspension decreased the viability of vascular endothelial cells at different times and exposure time groups of 12h and 24h showed significance statistically in comparison to the solvent control. Compared with the same concentration group of 12h and 6h, exposure time groups of 24h had statistically significant differences. Coal-fired PM2.5 could significantly decrease the viability of hman umbilical vein endothelial cells in dose-dependent and time-dependent manner. Vascular endothelial injury might be one of the possible mechanisms of cardiovascular toxicity caused by coal-fired PM2.5.
刘芳盈, 王菲菲, 丁明玉, 李杰. 燃煤细颗粒物对血管内皮细胞EA.hy926的细胞毒性[J]. 中国环境科学, 2012, 32(1): 156-161.
LIU Fang-Ying, WANG Fei-Fei, DING Ming-Yu, LI Jie. Cytotoxicity assessment of fine particles from coal combustion on EA. hy926 vascular endothelial cells. CHINA ENVIRONMENTAL SCIENCECE, 2012, 32(1): 156-161.
