Abstract：The carcinogenicity and toxicity of pentachlorophenol (PCP) exposure on the Chinese rare minnow (Gobiocypris rarus) embryos were investigated. G. rarus embryos at 0 hour past fertilization (hpf) were exposed to PCP at different concentrations (0, 7.5, 30, 60, 120, 250μg/L). DMSO(<0.01%,V/V) and 17a-ethynylestradiol (EE2, 2.5ng/L) were set as solvent control and positive control, respectively. The embryonic development was observed under the stereomicroscope. The hatching rate, relative survival rate at 96 hpf, and deformity rate through the entire embryos developmental process were calculated. Furthermore, the mRNA expressions of CYP1A and p53 gene were analyzed by semi-quantitatively RT-PCR. PCP exposure resulted in delayed embryonic development, condensation of embryonic eggs, formation of pericardial cysts, curvature of the spine or even death. The hatching rate and relative survival rate at 96 hpf were reduced while deformity rate were increased in a dose dependent manner of PCP. The mRNA level of CYP1A and p53 in embryos also were significantly up-regulated with increasing PCP concentration. PCP had significant toxic effects on G. rarus embryos. The hatching rate, relative survival rate at 96 hpf, malformation rate, CYP1A and p53 expression levels can be used as sensitive biomarkers to evaluate the toxic effects of PCP exposure on fish embryos.
引用本文:
熊力, 马永鹏, 毛思予, 苏永良, 金帮明, 刘堰. 五氯酚对稀有鮈鲫胚胎毒性效应研究[J]. 中国环境科学, 2012, 32(2): 337-344.
XIONG Li, MA Yong-Peng, MAO Si-Yu, SU Yong-Liang, JIN Bang-Ming, LIU Yan. Toxic effects of pentachlorophenol on the Chinese rare minnow embryos. CHINA ENVIRONMENTAL SCIENCECE, 2012, 32(2): 337-344.
