Abstract:Amount of Microcystin-LR in water was rapidly and quantitatively detected by SPR (surface plasmon resonance) technique. After the MC-LR being immobilized on the surface of the chip, the stability of the chip was tested, and the standard curve was constructed by the principle of competitive immune inhibition. The results showed that the relative standard deviation of 50 cycles was 0.62%, which represented the stability of the immobilized chip. And the limited concentration of detection is arrived at 0.25ng/ml.. For its stability and sensitivity, the SPR detection method would be applied to assay of MC-LR in the water.
周宏敏, 欧惠超, 任鹏, 张海燕, 王晓萍, 罗昭锋. 利用SPR技术测定湖水中微囊藻毒素[J]. 中国环境科学, 2012, 32(7): 1284-1287.
ZHOU Hong-Min, 欧Hui-Chao , REN Peng, ZHANG Hai-Yan, WANG Xiao-Ping, LUO Zhao-Feng. A novel detection method of microcystin of lake water by surface plasmon resonance technique. CHINA ENVIRONMENTAL SCIENCECE, 2012, 32(7): 1284-1287.