Abstract:The method of terminal restriction fragment length polymorphism (T-RFLP) was used to study the community structures and diversities of denitrifying bacteria and aerobic methanotrophs by two key functional genes nosZ and pmoA in the wetland soils of dry-up lake in the Inner Mongolia Plateau. And the effects of soil factors on them were also evaluated, then the mechanism of microbiological communities in wetland soils response to the dry-up lake were discussed. The results showed that the diversity indices of denitrifying bacteria and aerobic methanotrophs were higher, and community structures appeared more complicated at the later dry-up plots. The opposite results were shown at the earlier dry-up plot and the adjacent steppe. Their similarity coefficients gradually declined from the middle of the lake to the adjacent steppe, indicating communities dynamic succession. During drying process, soil moisture and organic matter content had significant influence on both of them. In addition, high pH value and high NH4+-N had inhibitory effects on denitrifying bacteria and the aerobic methanotrophs, respectively. The microbial communities were under the stress of dry-up process, which led to more emission of carbon dioxide and other greenhouse-gas from wetland soils.
白玉涛, 周玉, 赵吉. 内蒙古高原干涸湖泊反硝化及甲烷氧化细菌的群落分析[J]. 中国环境科学, 2012, 32(7): 1293-1301.
BAI Yu-Tao, ZHOU Yu, ZHAO Ji. Community structures of denitrifying bacteria and methanotrophs in wetland soils of dry-up lake in the Inner Mongolia Plateau. CHINA ENVIRONMENTAL SCIENCECE, 2012, 32(7): 1293-1301.