Abstract:The method for phosphorus speciation in sediment developed under the frame of Standard Measurements and Testing (SMT) Program of the European Commission is widely used, but it is hard to compare and evaluate the results of the analysis made by different operators. Phosphorus fraction in certified stream sediment reference material (GSD-12) was analyzed with the SMT method in this study. Owing to the accuracy and reproducibility of fractional phosphorus content in GSD-12 determined by SMT method, GSD-12 was suggested to be a reference material inserting in sediment phosphorus content analysis to fulfill the requirement of quality assurance and quality control during the measurement. Based on the comparison results from different phosphate analysis methods, and discussion of operational details in SMT procedure, the contents of TP, IP, OP, HCl-P and NaOH-P in GSD-12 were proposed as 156±5, 77±2, 39±3, 41±2, 42±2mg/kg, respectively. Phosphorus content in sediments from Lake Taihu was determined by GSD-12inserting using SMT method and showed satisfied results. Using reference materials inserting is helpful for making the results from SMT method more comparable.