Abstract：The surface and underground water of Sun Yat-sen University water cycle experiment base was sampled and analyzed by 16S rRNA gene analytical methods to acquire the data of microorganism communities’ distribution, then, the rarefaction curves and richness/diversity estimators were calculated and compared by Mothur software. Microbiological communities in groundwater were similar to those in surface water , with the predominant group of Proteobacterium, Firmicutes, Uncultured Archaeon(uncultured Crenarchaeote and Euryarchaeote). Proteobacteria made up 29.7%、30.3%、28.1%、20.9%、14.2% of the microbial communities from Shangyouyan, Xiayouyan, R1, R2, R3 , respectively. The same samples from Shangyouyan, Xiayouyan, R1, R2, R3 also consisted of 9.7%、20.3%、19.8%、8.6%、39.6% Firmicutes and 14.2%、12.3%、11.7%、12.1%、3.5% Archaea, respectively. On the other hand, the data of richness and diversity estimators such as Chao1, Ace, Shannon, and Simpson indicated that microorganism diversities were different in groundwater and surface water, which showed the difference among those microorganism’s characteristics. The study concluded that microorganism evolves in response to the change of the hydrogeological and ionic conditions that are influenced by the water environment and the recharging of water resources.
黄小兰, 陈建耀, 周世宁, 谢丽纯, 杨雪韵, 蒋华波, 董林垚. 小流域地下水与地表水微生物组成结构差异及其成因分析[J]. 中国环境科学, 2012, 32(9): 1647-1654.
HUANG Xiao-Lan, CHEN Jian-Yao, ZHOU Shi-Ning, XIE Li-Chun, YANG Xue-Yun, JIANG Hua-Bo, DONG Lin-Yao. Cause on the differences of microorganism between small watershed surface water and underground water. CHINA ENVIRONMENTAL SCIENCECE, 2012, 32(9): 1647-1654.
