Abstract:A halophilic bacterial consortium capable of degrading phenanthrene was developed from Shengli Oilfield in China. The diversity of naphthalene dioxygenase (ndo) genes in the bacterial consortium was analyzed by the method of clone library. Consortium consist of six nah-like genes. Three of them (covered 92.7% of the total clones) related to the classic nah-like genes with identity of about 89%. Using real-time reverse transcription PCR and other techniques, this study investigated the dynamic expression of ndo genes, biodegradation rate, biomass and bioavailability of phenanthrene in bacterial consortium growing at different salinities. As the salinity increased from 10% to 20%, the lag phase increased from 1 day to 3 days, the time needed for degrading 100mg/L phenanthrene increased from 6 days to 9 days. Both of the dissolved phenanthrene concentrations at 10% and 20% salinity increased constantly during the biodegradation, and One-way ANOVA(n=3, P>0.05) analysis showed there were no significant difference between them. A certain range of salinity probably not affected the bioavailability of phenanthrene. The expression of ndo genes at 20% salinity was lower than that at 10% salinity, while both of them decreased at the lag phase, and then increased constantly. Increasing salinity and high concentration of phenanthrene inhibited the expression of ndo genes.
何芬, 王立华, 宁大亮, 郭光, 王慧. 嗜盐菌群对菲的降解及萘双加氧酶基因的表达规律[J]. 中国环境科学, 2012, 32(9): 1662-1669.
HE Fen, WANG Li-Hua, NING Da-Liang, GUO Guang, WANG Hui. Phenanthrene biodegradation and dynamic change of expression of naphthalene dioxygenase (ndo) genes in a halophilic bacteria consortium. CHINA ENVIRONMENTAL SCIENCECE, 2012, 32(9): 1662-1669.