Abstract:This paper reports an approach to detect genotoxicity of characteristic pollutants using Comet Assay in the Bohai estuaries. Gobies, a tested organism were temporary reared in estuary and exposed to toxicant 48h. Collecting the Gobies’ peripheral blood cells is to detect its tail moment (TM), which is an indicator of DNA damage condition. And thus assess the risk of estuary genotoxicity. The simple, fast and high-sensitive method allows analyzing the damage condition by using Comet Assay quantitatively and indicates the characteristic pollutants of estuary may cause DNA damage of Gobies’ peripheral blood cell. Thus, the base-on-lab method of detecting estuary pollutants by Comet Assay is feasible and innovative.
洛昊, 梁斌, 马明辉, 张振冬, 张志峰. 彗星实验检测渤海主要入海河流遗传毒性[J]. 中国环境科学, 2013, 33(1): 161-165.
LUO Hao, LIANG Bin, MA Ming-Hui, ZHANG Zhen-Dong, ZHANG Zhi-Feng. Evaluation and detection of genotoxicity in main estuaries of Bohai Rim by comet assay.. CHINA ENVIRONMENTAL SCIENCECE, 2013, 33(1): 161-165.