Application of luminescent bacteria and statistic method in groundwater quality assessment: A case study in coastal catchments in the Pearl River Delta
Abstract：Water samples from 8 domestic wells (Tangjia town), 6 observation wells (Zhuhai campus of SunYatsen University) and 1 from seawater as well in coastal catchments of Zhuhai City in the Pearl River Delta were selected as research objects. Water quality under natural and urbanized conditions was evaluated by using luminescent bacteria (Vibrio ficheri) and principal component analysis (PCA) of chemical parameters. The results showed that the primary cations of 15 samples were K+ and Na+, accounting for an averaged 71.55% of major cations. Major anions for domestic (T1~T8) and observation(D1~D6) wells were HCO3- (53.84%) and Cl- (66.37%), respectively. All samples except T3, T4 and D3 had inhibitory effect on luminescent bacteria, e.g. inhibitory ratio of D2 and D4 exceeded 30%, which can be considered as moderate toxic. Seawater stimulated luminescence and achieved a relative luminous inhibition of -12.45% within 30 min. General score of groundwater samples were calculated by extracting three principal components of PCA, yielding high scores at D2~D4 (unconfined aquifer) and low scores at D5 (confined aquifer), D6 (fissured aquifer) and D1 (unconfined aquifer) in the discharge area. In the comparative study catchment in Tangjia, the highest score was obtained from T1(17.92m below surface) and much low scores from the other domestic wells(T2~T8) with well depth less than 10m. A negative correlation (P<0.05) between relative luminous inhibition and the first component was identified, and 75% accuracy with zero “false negative” sample regarding toxicity prediction was obtained by fitting equation.
高磊, 陈建耀, 柯志庭, 王江, 谢丽纯, 杨雪韵. 采用发光菌和统计法评估地下水水质 ——以“珠江三角洲”滨海小流域为例[J]. 中国环境科学, 2013, 33(4): 623-630.
GAO Lei, CHEN Jian-Yao, KE Zhi-Ting, WANG Jiang, XIE Li-Chun, YANG Xue-Yun. Application of luminescent bacteria and statistic method in groundwater quality assessment: A case study in coastal catchments in the Pearl River Delta. CHINA ENVIRONMENTAL SCIENCECE, 2013, 33(4): 623-630.
