Abstract：A tylosin-degrading strain TS1 was isolated from the soil deposited by tylosin pharmaceutical waste. The cells of the isolate were Gram-negative rods and the colony morphology was round, oyster white, opaque, and smooth with regular edge. Strain TS1 was capable of degrading 100% of tylosin in medium with an initial concentration of 100 mg/L after 5days of incubation. The tylosin degradation by the strain TS1 followed a first-order kinetics model. For tylosin with the initial concentrations of 50, 100, 200, 300, 400, 500mg/L, the degradation equations were lnc =﹣0.4078 t +4.043, lnc = ﹣0.4496 t +4.8416, lnc =﹣0.4069 t +5.4932, lnc =﹣0.4174 t +5.9766, lnc =﹣0.4233 t + 6.2483, and lnc = ﹣0.342 t + 6.4618, and the half-life periods (t1/2) were 1.69 , 1.54 , 1.70 , 1.66 , 1.64 , 2.03 d, respectively. When the peptone or NH4+-N was used as co-metabolism substrate with tylosin, strain TS1 could degrade above 99% of tylosin in medium with an initial concentration of 300mg/L after incubation for 7days. The kinetics equations of degradation were lnc =﹣0.4174 t +5.9766 and lnc =﹣0.3719t + 6.0133, and t1/2 were 1.66d and 1.86d, respectively. However, only 66.7% of tylosin was degraded by the strain TS1 after 10days of incubation when glucose was selected as co-metabolism substrate, and t1/2 was also extended to 6.44days, suggesting that glucose may have a negative action on the tylosin degradation by the strain TS1.
孙瑞珠, 马玉龙, 张娟, 张作义, 王艳, 王敏, 马志强. 泰乐菌素降解菌的筛选及其降解动力学研究[J]. 中国环境科学, 2013, 33(4): 722-727.
SUN Rui-Zhu, MA Yu-Long, ZHANG Juan, ZHANG Zuo-Yi, WANG Yan, WANG Min, MA Zhi-Qiang. Isolation and degradation dynamics of a tylosin-degrading strain. CHINA ENVIRONMENTAL SCIENCECE, 2013, 33(4): 722-727.
