Abstract:Three surfactants, anioic docusate sodium salt (AOT),cationic cetylrimethylammonium bromide(CTAB) and anioic biosurfactant rhamnolipid(RL),were used to construct the reverse micelles system in (n-Hexanol/ 2,2,4-Trimethylpentane). The optimal extraction conditions of cellulase through different reverse micelles were studied. Several important parameters affecting the cellulase extraction efficiency were investigated, such as aqueous pH, surfactant concentration, ionic strength and salt species. It was shown that the optimal aqueous phase pH value for both AOT and RL extractants (anionic surfactant) was 3.0, while it was 8.0 for extractants of cationic surfactant CTAB. The optimal surfactant concentrations were 23.7mmol/L, 15mmol/L and 2.75mmol/L for AOT, CTAB and RL, respectively. Salt NaCl affected the extraction efficiency. For all the three surfactants, the extraction efficiency decreased with increase of NaCl concentration in aqueous phase. Moreover, ions species in the aqueous phase affected the extraction efficiency. The optimal extraction efficiency of cellulase varies with the type of reverse micelles (CTAB>AOT>RL).