Abstract:The main factors which affected the catalytic oxidation of veratryl alcohol (VA) by lignin peroxidase(Lip) entrapped in rhamnolipid(RL) reverse micellar or micellar systems were investigated. Meanwhile, the partition coefficient of insoluble substrate VA in the heterogeneous reverse micellar medium were studied. It was shown that Lip hosted in RL/isooctane-n-hexanol(1:1,V:V)/water reverse micellar system could express higher enzymatic activity under the following conditions: pH 3.8, [RL]=10mmol/L, w0=15.0, [H2O2]=74μmol/L. With respect to the RL micellar system, the optimum values were as follows: pH 3.4, [RL]=0.012mmol/L, [H2O2]=2.45mmol/L. In optimum catalytic conditions, the half-life of Lip was 40h, and the catalytic activity in reverse micelle was 2.86 times than that in micellar system. Based on the biphasic model and the corresponding kinetic method, a conclusion could be made that VA was mainly solubilized in the pseudophase of reverse micelle, and the partition coefficient of VA between the pseudophase and organic solvent phase was 70.4. The determination of partition coefficient is important for further discussing the kinetic mechanism of Lip-catalyed oxidation VA in the RL reverse micellar system.