Abstract：：To obtain new whole cell biosensor which can be used for rapidly detecting metal ion pollution in the environment, Tetrahymena thermophilia was used in this study. Luciferase (LUC) gene was recombinated into the vector pBX containing Tetrahymenna Metallothioneins gene MTT1 promoter and tubulin terminator. Plasmid pBX–LUC was constructed and transformed into Tetrahymena strain by particle bombardment. Then, the LUC gene replaced the Tetrahymena macronuclear MTT1 gene by homologous recombination under increasing paromomycin. The B2086-LUC strain was obtained. The B2086-LUC strain is sensitively responsed to heavy metals cadmium and mercury ion (5 ~ 10 ng/mL) and less sensitive for copper and zinc ion (0.5~1 mg/mL). The results showed genetically engineered whole cell biosensor Tetrahymena B2086-LUC strain can be used for rapidly detecting cadmium and mercury pollution in environment.
张鹏幸, 许静, 卢剑功, 王伟. 含荧光素酶四膜虫B2086-LUC全细胞生物传感器的构建及其对重金属离子的响应[J]. 中国环境科学, 2013, 33(6): 1075-1080.
ZHANG Peng-Xing, XU Jing, LU Jian-Gong, WANG Wei. Construction of whole cell biosensor Tetrahymena B2086-LUC containing luciferase and its response to heavy metal ion.. CHINA ENVIRONMENTAL SCIENCECE, 2013, 33(6): 1075-1080.
