Abstract：Kunming mice were randomly grouped into six groups and orally administered with drugs daily for a week; the groups included one solvent control group, three cypermethrin groups, one high dose cypermethrin plus vitamin E protection group and one vitamin E group. The exposure doses of cypermethrin groups were 10, 20and 40mg/kg respectively. Some brain tissues were then made into homogenates for the measurement of ROS (reactive oxygen species), GSH (glutathione) and MDA (Malondialdehyde) contents. Meanwhile, DPC (DNA-protein Crosslink) coefficients were detected from brain cell suspension. The brain contents of ROS, MDA and DPC coefficients increased gradually in a dose-dependent manner, whereas GSH content decreased accordingly. In the exposure group with the dose of 20mg/kg, ROS(841.3±100.34), GSH contents[(12.54±1.316)nmol/L] and DPC coefficients(0.054±0.004) were significantly higher than those of the control group (P<0.05); In the highest dose group (40mg/kg), GSH content [(10.51±1.545)nmol/L]was significantly higher than that of the control group (P<0.05), the significance of differences of ROS(1014.3±81.67)、MDA contents[(2.849±0.218)μmol/L] and DPC coefficients(0.079±0.005) to the control group have all become extreme (P<0.01). However, compared to the high cypermethrin dose group, high dose cypermethrin plus vitamin E protection group had lower ROS(719.5±74.56 )(P<0.05), MDA[(1.662±0.265)μmol/L] (P<0.01) and DPC coefficients(0.055±0.005) (P<0.05) levels, and higher GSH contents[(16.52±1.985)nmol/L](P<0.05). Cyermethrin at certain doses (320mg/kg) can induce oxidative stress in mice brain, whereas vitamin E has antioxidant effects.
马萍, 张忠杰, 焦铭, 单士刚, 武阳, 陈姣娥, 杨旭. 氯氰菊酯对小鼠脑细胞的氧化损伤及维生素E的抗氧化作用[J]. 中国环境科学, 2013, 33(7): 1323-1327.
MA Ping, ZHANG Zhong-Jie, JIAO Ming, DAN Shi-Gang, WU Yang, CHEN Jiao-E, YANG Xu. Oxidative damage of pesticide cypermethrin on mouse brain cells and the antioxidant role of vitamin E. CHINA ENVIRONMENTAL SCIENCECE, 2013, 33(7): 1323-1327.
