Abstract:In order to explore whether the inhaled formaldehyde have toxic effect on animal bone marrow tissue and induce changes in its gene expression profile, certain mice proto-oncogene and anti-oncogene were selected as the target in this study. SPF grade balb/c male mice were exposed to 0, 0.5and 3.0mg/m3 formaldehyde by inhaling, respectively, for two weeks in a simulated occupational exposure mode (2-day interval between each 5-day exposure). After exposure, bone tissue was employed for the determination in the change of expression profile for c-myc, MDM2 and p53with semi-quantitative RT-PCR. Compared with the control group, the expression profile for c-myc, MDM2 and p53 all changed under different concentration of formaldehyde exposure. Especially in the mice group exposed to 3.0mg/m3 formaldehyde, there appears a significant difference (P<0.05) in that MDM2 gene expression is up-regulated while p53gene expression is down-regulated. Inhaled formaldehyde is toxic to animal bone marrow tissue and will cause the change in its gene expression profile.
李慧, 张玉超, 柯玉洁, 秦晓丹, 刘旭东, 问华肖, 熊棣, 李睿, 杨旭, 丁书茂. 甲醛对原癌基因c-myc、MDM2及抑癌基因p53的影响[J]. 中国环境科学, 2013, 33(8): 1483-1486.
LI Hui, ZHANG Yu-Chao, KE Yu-Jie, QIN Xiao-Dan, LIU Xu-Dong, WEN Hua-Xiao, XIONG Di, LI Rui, YANG Xu, DING Shu-Mao. Expression of proto-oncogene c-myc, MDM2 and anti-oncogene p53 induced by different concentrations of formaldehyde. CHINA ENVIRONMENTAL SCIENCECE, 2013, 33(8): 1483-1486.