Abstract:Pyrogallic acid (PA) secreted by submerged macrophytes Myriophyllum spicatum is a potent allelochemical toward Microcystis aeruginosa. For studying the mechanism of the allelopathic effect, the intracellular O2?? level, H2O2/ OH? level, SOD activity, MDA level and percentage of membrane-injured cells were detected, after M.aeruginosa were exposed to PA for 12h under dark and light conditions respectively. The results showed that 0.5mg/L PA could induce generation of O2?? significantly (P<0.01); H2O2/OH? level and SOD activity both exhibited dose-dependent increases; and to MDA level and Prodium Iodide fluorescence(indication of membrane-injured cells), significant increases were observed when exposed to 0.5and 1mg/L PA correspondingly(P<0.05). The results indicated that a low concentration of PA could induce significant ROS generation and consequent lipid peroxidation and membrane injury in M.aeruginosa, which suggested that oxidative damage may be one of the important allelopathic mechanisms.
孙雪梅, 刘碧云, 鲁志营, 吴振斌. 焦性没食子酸对铜绿微囊藻的氧化胁迫效应研究[J]. 中国环境科学, 2013, 33(10): 1835-1841.
SUN Xue-Mei, LIU Bi-Yun, LU Zhi-Ying, WU Zhen-Bin. Study on oxidative damage to Microcystis aeruginosa mediated by allelochemical pyrogallic acid from submerged macrophytes. CHINA ENVIRONMENTAL SCIENCECE, 2013, 33(10): 1835-1841.