Abstract：A selection of a chlorothalonil-degrading strains was bacteria CTN-4 (Pseudomonas sp.) which had been selected and could use chlorothalonil as the sole carbon source. The objective of this study was to determine the optimum conditions for the biodegradation of chlorothalonil by Pseudomonas sp., and to establish a kinetic model to simulate the degradation process. Response surface methodology (RSM) based on CCD experimental design was employed to determine the optimum conditions. Results indicated that the optimum conditions for chlorothalonil degradation based on RSM and contour plots were as follows: concentration of glucose, 0.40% (w); initial concentration and pH of chlorothalonil were 102.66mg/L and 7.05, respectively. In addition, the predicted value of chlorothalonil degradation rate under the optimum conditions was 93.89%, which was in good agreement with the experimental result: under the optimum conditions, the degradation rate of chlorothalonil was 95.07%. Moreover, experiments show that the effects of pH, temperature and the initial substrate concentration on the degradation of chlorothalonil were obvious. And the degradation kinetics curves of chlorothalonil can be fitted by the modified Gompertz model which is similar with the process of chlorothalonil degradation.
王光利, 李新风, 张辉, 熊明华, 李峰. CTN-4降解百菌清的条件优化及动力学模型的研究[J]. 中国环境科学, 2013, 33(11): 1999-2005.
WANG Guang-Li, LI Xin-Feng, ZHANG Hui, XIONG Ming-Hua, LI Feng. Optimization of CTN-4to chlorothalonil-degrading conditions and a kinetics model. CHINA ENVIRONMENTAL SCIENCECE, 2013, 33(11): 1999-2005.
