Abstract:Ultrasonic on inactivation of Cryptosporidium parvum was studied under different operational parameters, such as ultrasonic frequency, ultrasonic power, pH and temperature. The mechanism of inactivation of Cryptosporidium parvum was preliminary discussed by morphological observation, and inactivating kinetic analysis was also focused. The results indicated that low frequency favored the inactivation of Cryptosporidium parvum, 92.5% of inactivating rate could be achieved under the condition of 19.8kHz ultrasonic (15min), pH 7.2and temperature 20±1℃, while the inactivating rate decreased if the frequency increased. Furthermore, the inactivating rates were almost the same regardless of ultrasonic power was 103W or 151W, and the value of pH had slight effect on inactivating. The inactivating rate was 95.6% using ultrasonic treatment for 15min at 36℃ while 88.3% at 9℃, which indicated higher temperature was conducive to the inactivation of Cryptosporidium parvum. Ultrasonic cavitation caused cell membrane damage and cytoplasm outflow in inactivating Cryptosporidium parvum. Ultrasonic inactivation of Cryptosporidium parvum was in line with pseudo-first-order kinetics, and lower frequency and higher power lead to better ultrasonic cavitation. Therefore, it was suggested that the intensity of ultrasonic cavitation was the main factor for inactivating Cryptosporidium parvum.
胡健龙, 冉治霖, 李绍峰, 张朝升. 超声灭活饮用水中隐孢子虫研究[J]. 中国环境科学, 2014, 34(2): 431-436.
HU Jian-Long, RAN Zhi-Lin, LI Shao-Feng, ZHANG Chao-Sheng. Inactivation of Cryptosporidium parvum in drinking water by ultrasonic irradiation. CHINA ENVIRONMENTAL SCIENCECE, 2014, 34(2): 431-436.