Abstract:A phenol-degrading strain was isolated from the activated sludge of a cokes wastewater treatment facility in Taiyuan City. Based on its physiology, biochemical characters and the analysis of its 16S rRNA gene sequence, the isolated strain named PD-07 was identified as a member of the genus Diaphorobacter. Metabolic pathway research showed that the strain could be induced to synthesize intracellular catechol-2,3-dioxygenase.To improve the phenol degradation rate, a study of embedding immobilized strain was conducted with calcium alginate as material. Firstly, factors playing important roles in the phenol degradation rate of immobilized cell were selected based on Plackett-Burman design. Then the path of steepest ascent was undertaken to approach the optimal region of the phenol degradation rate. Finally, the optimal levels of those main factors were further optimized by using Box-Behnken design and response surface analysis. Quadratic equation was adopted to fit the test data. The fitting curve had a good correlation with the measured values. The optimal conditions were as follows: alginate concentration 3.83%(m/V), calcium chloride concentration 0.3mol/L, bacteria-cement ratio 1:26.73, immobilized time 2h, rotation rate 180r/min, culture temperature 30℃, initial pH value 7.2, liquid-solid ratio 4.86:1. Under the optimal conditions mentioned above, the phenol degradation rate reached 96.89%.
葛启隆, 岳秀萍, 王国英. 一株苯酚降解菌的分离鉴定及响应面法优化其固定化[J]. 中国环境科学, 2014, 34(2): 518-525.
GE Qi-Long, YUE Xiu-Ping, WANG Guo-Ying. Isolation and identification of a phenol-degrading strain and optimization for its immobilization using response surface methodology. CHINA ENVIRONMENTAL SCIENCECE, 2014, 34(2): 518-525.