Abstract：With five microalgae (dinoflagellates, diatoms, green algae species) as test materials, the effect of different nutrient conditions had been explored on the stable carbon and nitrogen isotopic throughout the growth phases. The result showed that among different classes of microalgae the d13C and d15N at the exponential phase were lower than those at the stable phase, indicating no correlation with growth velosity (P>0.05). Bacillariophyceae presented a higher d13C than dinophyceae and chlorophyceae, while the d13C of dinophyceae was nearly identical to that of chlorophyceae. In terms of d15N, the dinophyceae falls behind both bacillariophyceae and chlorophyceae, with bacillariophyceae approximating to chlorophyceae. In addition, significant d15N differences have been discovered among Prorocentrum micans, Alexandrium tamarense and Amphidinium carterae Hulburt. In this study, all five classes were grown under three distinctive cultures—nutrient sufficiency, nitrogen deficiency and phosphorus deficiency; either nitrogen or phosphorus limitation contributed to a more positive d13C and d15N, yet less obvious effect was observed in phosphorus-deficiency culture than in nitrogen-deficiency condition.
王海霞, 刘瑀, 关春江, 路琳, 鲍惠铭, 刘保占. 营养条件对微藻碳、氮稳定同位素组成的影响[J]. 中国环境科学, 2014, 34(3): 727-733.
WANG Hai-Xia, LIU Yu, GUAN Chun-Jiang, LU Lin, BAO Hui-Ming, LIU Bao-Zhan. Effects of nutritional conditions on the stable carbon and nitrogen isotope of microalgae. CHINA ENVIRONMENTAL SCIENCECE, 2014, 34(3): 727-733.
