Abstract：To explore the effects of different compositions of fine particles on the cardiovascular system. Coal sample taking Datong coal as a sample, Coal-fired PM2.5 was sampled by fixed source dilution channel in the laboratory. All particles, as well as isolated inorganic and organic compositions, were extracted to contaminate EA.hy926cells for 24h respectively. To evaluate oxidative damages of different compositions of coal-fired PM2.5 exposed to the EA.hy926cells, the assays of superoxide dismutase (SOD), malondialdehyde (MDA), Glutathione peroxidase (GSH-Px) were carried out using assay kits according to the manufacturer’s instructions. The results showed that as the dose increased, SOD activity decreased in EA.hy926exposed to different compositions of coal-fired PM2.5 24h. Each dose group was statistically different (P inorganic compositions > all particles, with statistically significance differences among the same dose. Compared to solvent control, as the dose increased, GSH-Px activity decreased in EA.hy926exposed to different compositions of coal-fired PM2.5. At the same dose, GSH-Px inhibition of different compositions was: inorganic compounds > organic compositions > all particles, with no statistically significance differences. As the dose increased, MDA levels in supernatants treated with all particles, inorganic compositions and organic compositions increased, respectively. At the low doses, MDA level of different compositions of coal-fired PM2.5 on EA.hy926was: organic compositions > all particles > inorganic compositions, while at the high doses : all particles > inorganic compositions > organic compositions with statistically significance, and as the dose increased, MDA level caused by all particles and inorganic compositions increased significantly, while changes of organic compositions is leveling off. The results can be seen from the above, different compositions of PM2.5exerted oxidative damage to EA.hy926 ,reduced activities of SOD, GSH-Px, and increased MDA level. But the mechanisms underlying them need futher exploration.
