Abstract:Phosphorus removal sludge with nitrate, nitrite and oxygen as the sole electron acceptor were acclimatized in three parallel sequencing batch reactors, namely RN03, RN02 and R02 respectively. Operation results showed that phosphorus removal rates were 84.8%, 87.4% and 78.7% on average for RN03, RN02 and R02, with effluent TP concentration of 0.758mg/L, 0.931mg/L and 0.632mg/L. High-throughput technology was employed to evaluate the microbial similarity and community structure of phosphorus removal sludge with different electron acceptors. Results indicated that denitrifying phosphorus removal sludge with nitrate and nitrite as electron acceptors exhibited similar bacterial community structure and was distinct from that of aerobic phosphorus removal sludge. The dominant microbial organisms were clustered into five clades based on the phylogenetic relationship of the significant OTUs sequences. Through sequence BLAST, 0.716%, 1.78%, 2.53% and 4.80% of sequences from Seed, RN03, RN02 and R02 could be assigned to Accumulibacter-like sequences at similarity level of 97%; 0.368%, 1.44%, 1.32% and 30.9% of sequences could be assigned to GAO-like sequences, indicating that alternating anaerobic-anoxic condition was favorable toward elimination of GAO. Moreover, potential public health hazard was revealed for nitrite based denitrifying phosphorus removal sludge.
吕小梅, 李继, 李朝林, 刘洞阳, 邵明非, 夏雪. 不同电子受体除磷污泥相似性与菌群结构研究[J]. 中国环境科学, 2014, 34(4): 935-941.
吕Xiao-Mei , LI Ji, LI Chao-Lin, LIU Dong-Yang, SHAO Ming-Fei, XIA Xue. Microbial similarity and community structure of phosphorus removal sludge with different electron acceptors. CHINA ENVIRONMENTAL SCIENCECE, 2014, 34(4): 935-941.