Abstract：To examine the changes of ammonia-oxidizing microorganisms community in an iron mine of the upstream area of Miyun Reservoir, soil samples were collected from fourteen sites. Quantification of ammonia-oxidizing bacteria (AOB) and archaea (AOA) were performed by real-time PCR, and the microbial community structures were studied by denaturing gradient gel electrophoresis (DGGE). The results showed that the copy numbers per gram of dry soil of AOB changed from 3.01×107to 1.08×109and the copy numbers per gram of dry soil of AOA varied from 8.65×107to 2.69×109. Pearson correlation and the redundancy analysis results showed that heavy metal pollution had changed the abundance and community structure of soil ammonia-oxidizing microorganisms in this iron area. The Cu pollution had a significant inhibitory effect on abundance of AOA (r = -0.653*, P < 0.05), but had no effect on AOB; The Zn pollution had a remarkable effect on AOA/AOB ratio of tailing area (r= -0.606*, P<0.05); Cd pollution changed the AOB community structure and reduced its diversity; The long-term interference of Cr did not change the abundance and community structure of soil ammonia oxidizing microorganisms, but inhibited the ammonia oxidation rate obviously. The results showed that heavy metal pollution affect the circulation of nitrogen in soil ecosystem to a certain extent.
洪晨, 邢奕, 司艳晓, 李洋. 铁矿区内重金属对土壤氨氧化微生物群落组成的影响[J]. 中国环境科学, 2014, 34(5): 1212-1221.
HONG Chen, XING Yi, SI Yan-Xiao, LI Yang. Impact of long-term heavy metals pollution on ammonia oxidizing microbial community in an iron mine soil. CHINA ENVIRONMENTAL SCIENCECE, 2014, 34(5): 1212-1221.
