Abstract:Variations of soluble microbial products (SMP) and extracellular polymeric substances (EPS) extracted from MBR sludge under different salinity shock were studied using excitation-emission matrix fluorescence spectroscopy. Analysis of the EEM spectra showed that the fluorescence intensity of tryptophan protein-like peak B (270nm, 350nm), humic-acid-like Peak C (375, 475nm), fulvic-acid-like Peak D (260, 460nm) in the EEM spectra of SMP and Peak B in the EEM spectra of EPS increased as salinity shock increased. Peak C in the EEM spectra of EPS decreased as salinity shock increased when salinity shock was over 2.5g/L. Four components were identified from the EEMs by PARAFAC, which were protein-like C1 (230/280, 350nm), humic-acid-like C2 (290/310, 380nm), C3 (290/360, 460nm) and C4 (270/340, 440nm), respectively. When the salinity shock was over 2.5g/L, the fluorescence intensity of C1, C2 and C3 in SMP were positively correlated with the salinity shock. The fluorescence intensity of C3 and C4 in EPS were positively and negatively correlated (with correlation coefficients30.90) with the salinity shock, respectively. The total fluorescence intensity of the protein-like component and humic acid-like component had positive correlations with humic acid and protein content determined by chemical methods (with correlation coefficients>0.93) which can be used for the quantitative calculation. However, there were no obvious correlations in EPS.
安莹, 王志伟, 李彬, 韩小蒙, 吴志超. 盐度冲击下MBR污泥SMP和EPS的三维荧光光谱解析[J]. 中国环境科学, 2014, 34(7): 1754-1762.
AN Ying, WANG Zhi-Wei, LI Bin, HAN Xiao-Meng, WU Zhi-Chao. Analysis of the EEM Fluorescence Spectra of the SMP and EPS in MBR sludge under salinity shock. CHINA ENVIRONMENTAL SCIENCECE, 2014, 34(7): 1754-1762.