Abstract：To study the diversity of cultured microorganisms from oil shale samples in Fushun west open pit mine, cultured microorganisms were isolated and purified by dilution-plate method, and then were identified and phylogenetically analyzed by 16S rDNA sequences (bacteria) and ITS-rDNA sequences (fungi). The results showed that the 8 bacterial isolates from nutrient agar medium belonged to 5 genera (Bacillus, Lysinibacillus, Brevibacillus, Paenibacillus and Rhodococcus), and the 4 fungal isolates from potato dextrose agar medium belonged to 4 genera (Cladosporium, Aspergillus, Absidia and Eupenicillium). 2 bacterial strains (ZK4 and ZK5) could grow well on the agar medium with oil shale as the sole carbon and nitrogen source for more than 5 generations, and were further considered as potential strains for the biotransformation of oil shale.
蒋绍妍, 王文星, 薛向欣. 抚顺西露天油页岩可培养微生物的分离与鉴定[J]. 中国环境科学, 2014, 34(8): 2126-2132.
JIANG Shao-Yan, WANG Wen-Xing, XUE Xiang-Xin. Isolation and identification of cultivable microorganisms in oil shale from Fushun western open group, China. . CHINA ENVIRONMENTAL SCIENCECE, 2014, 34(8): 2126-2132.
