Abstract:Functional diversity of carbon-source metabolism of oil shale microorganisms in Fushun Basin Western Open Group was investigated by Biolog-GN2 and GP2 microplates method. The microbial community level physiological profiles (CLPP) were determined during 7 consecutive days. According to different chemical groups, the metabolic capacity and differences of microbial communities on different carbon sources were analyzed. The results showed that the metabolic level and metabolic rate of microbial communities on different carbon sources were obviously different. For the Gram-negative or Gram-positive bacteria communities, the utilization of carbon sources with amino group was the highest, followed by carboxylic acid, sugar and its derivatives, and sugar phosphates was the lowest. The analyses of Shannon-Wiener, Simpson and McIntosh indexes through multi-point calculation indicated that the carbon metabolic diversity of microbial communities in oil shale was generally high, but the diversity slightly decreased with incubation time.