Abstract：Water reclamation and reuse have been actively promoted in Beijing, but the potential influences of reclaimed water on the structure and function of microbial community were still poorly understood. Therefore, communities of both bacterial and ammonia oxidizing bacterias in cattail rhizosphere between the samples of reclaimed water outfall (RWO) in the Wang ping Wetland of Yong ding River, Beijing and far from the reclaimed water outfall (FRWO) were compared. Terminal Restriction Fragment Length Polymorphism (T-RFLP) was conducted to quantitatively detect the changes of bacterial and ammonia bacterial community structures and diversity. Then, Microbial Community Analysis Ⅲ, Canonical Correspondence Analysis (CCA) and Factor Analysis (FA) were used to investigate the changes of bacterial and ammonia bacterial community functions in different water qualities. The results were as followed: samples were classified according to the disturbance degree of reclaimed water. The bacterial diversity of RWO was significantly lower than that of FRWO, while the ammonia oxidizing bacterial diversity of RWO was higher than that of FRWO. As for bacteria, the function of root-attached phylotypes in RWO samples, which accounted for 55.6% of the bacterial community, was similar to the Sphingomonas sp. by 150-bp T-RFs. This function might be closely related to the total organic carbon in the biological chemical cycle. The major phylotypes from upstream samples had the same function with Pseudomonas sp.and Geitlerinema sp. by 80-bp T-RFs, whose proportion was 75.5% of the bacterial community, and the function was closely related to nitrogen in the biological chemical cycle. The major phylotypes from downstream samples had the same function with Micromonospora sp.by138-bp T-RFs, whose proportion was 68.7% of the bacterial community, and the function was closely related to metals such as Cu, V and Ti in the biochemical cycle. For ammonia oxidizing bacteria, the function of root-attached phylotypes in RWO sample, which accounted for 65.5% of all the ammonium bacterial community, was similar to the Nitrosomonas sp. by 266-bpT-RFsliving in high ammonium environment. The major phylotypes from FRWO had the same function with Nitrosospira sp. by 58-bp T-RFs living in low ammonium environment.
马栋山, 熊薇, 张琼琼, 郭羿宏, 赵文吉, 郭逍宇. 基于T-RFLP和因子分析的香蒲根际细菌群落研究[J]. 中国环境科学, 2014, 34(10): 2684-2691.
MA Dong-Shan, XIONG Wei, ZHANG Qiong-Qiong, GUO Yi-Hong, ZHAO Wen-Ji, GUO Xiao-Yu. The research of cattail rhizosphere bacteria community based on T-RFLP and FA. CHINA ENVIRONMENTAL SCIENCECE, 2014, 34(10): 2684-2691.
