Abstract:Airborne microbes are major biological components of the ecosystem and have important ecological functions. They affect human health, cause air pollution, and are key indicators of air quality. To determine the community structure of airborne microbes in a surface-flow wetland system constructed for sewage treatment in spring, the airborne bacteria and airborne fungi were analyzed by a 16S/18S rDNA gene clone library method. Phylogenetic analysis of airborne bacteria in the airborne microbes sample showed that there were seven groups: Proteobacteria, Actinobacteria, Planctomycetes, Cyanophyta, Chloroflexi, Bacteroidetes and Firmicutes. The main types of airborne bacteria were Betaproteobacteria (71.04%), Gammaproteobacteria (12.03%), Alphaproteobacteria (3.83%), Cyanophyceae (4.38%), Bacilli (3.28%) and Sphingobacteriales (2.19%), while the dominant genera of airborne bacteria included Massilia (66.66%), Pseudomonas (4.37%), Cyanothece (3.83%) and Serratia (3.28%). Phylogenetic analysis of airborne fungi in the airborne microbes sample showed that the main types were Dothideomycetes (61.18%), Zygomycetes (16.47%) and Discomycetes (14.12%). The dominant genera of airborne fungi included Pyrenophora (48.31%), Mortierella (15.7%), Cheilymenia (12.4%) and Boothiomyces (4.5%). Escherichia coli, Salmonella spp. and Clostridium perfringens were not detected in the sample of airborne microbe while other pathogens or conditioned pathogens, such as S. marcescens, p. putida, and Staphylococcus epidermidis were detected.
吴等等, 宋志文, 王琳, 徐爱玲, 夏岩. 人工湿地污水处理系统春季空气微生物群落结构分析?[J]. 中国环境科学, 2014, 34(12): 3164-3174.
WU Deng-Deng, SONG Zhi-Wen, WANG Lin, XU Ai-Ling, XIA Yan. Community structure of airborne microbes in spring in a constructed wetland system for sewage treatment.. CHINA ENVIRONMENTAL SCIENCECE, 2014, 34(12): 3164-3174.