Abstract:A highly efficient methyl orange-decolorizing strain CV-v was isolated in Wenzhou, Zhejiang. Results of 16S rRNA gene analysis indicated that this strain belongs to the Enterobacter genus. Data from one-factor-a-time experiments showed that methyl orange decolorization rate by the strain could reach over 80% after 48h at pH 5.0~9.0. The optimal temperature for decolorization was between 30~40oC. Among the tested carbon and nitrogen sources, glucose, maltose, beef extract, and yeast extract could significantly enhance the decolorization. Besides, Ca2+ and Mg2+ could also significantly enhance the decolorization. Meanwhile, almost completely decolorization was observed after 48h when the inoculums size reached to 7% (V:V). Moreover, the results of response surface design revealed that the optimal conditions for decolorization were pH 8.0, 1.7g/L glucose, 3.0g/L yeast extract, 3.0mmol/L MgCl2, and at 35.9oC. Finally, the results of verification tests indicated that methyl orange decolorization by the strain could reach 88.0% after 10h under the optimal conditions. In general, this strain has a great potential in azo dye decolorization.
都林娜, 李刚, 卢晓明, 闫成进, 卢圣佑, 泮琇, 陈文华. Enterobacter sp. CV-v对甲基橙的脱色特性与条件优化[J]. 中国环境科学, 2014, 34(12): 3175-3181.
DU Lin-Na, LI Gang, LU Xiao-Ming, YAN Cheng-Jin, LU Sheng-You, PAN Xiu, CHEN Wen-Hua. Characteristics of methyl orange decolorization by Enterobacter sp. CV-v and parameter optimization. CHINA ENVIRONMENTAL SCIENCECE, 2014, 34(12): 3175-3181.