Abstract:To examine the spatial changes of bacterial community in a copper mine tailing of Hubei, 14soil samples were collected, including 12 tailing samples and 3 compared samples (one from cultivated land ,two from another small copper mine tailing). DGGE (denaturing gradient gel electrophoresis) was employed to analyze V3~V6variable regions of bacterial 16S rRNA. Pearson correlation analysis and redundancy analysis (RDA) between bacterial community data and physicochemical parameters were carried out respectively to reveal relationships of the bacterial community structure, physical and chemical factors of the total samples, and contents of Cu, Zn. The results showed serious copper and zinc pollution, and there were significant differences for contents of Cu, Zn correlated with different physico~chemical characteristics of tailing samples, notably the significant position correlation [R=0.668(P<0.01)] between the content of Zn and organic matter. According to analysis of gene separated by DGGE the bacterial structures were different between samples. Overall, there was certain rule in the samples that low bacterial diversity, high similarity (the lowest similarity 53.1%) and relatively stable dominant bacteria. RDA demonstrated that the content of Cu, Zn influenced on bacteria community, Cu can inhibit microbial population, so can Zn, but in a certain concentration range, Zn may contribute to the development of microbial diversity, that is not laboratory studied simply linear relationship with their concentrations.
林海, 崔轩, 董颖博, 张国华, 李笑晴, 季振. 铜尾矿库重金属Cu、Zn对细菌群落结构的影响[J]. 中国环境科学, 2014, 34(12): 3182-3188.
LIN Hai, CUI Xuan, DONG Ying-Bo, ZHANG Guo-Hua, LI Xiao-Qing, JI Zhen. Impact on bacterial community structure of heavy mental of Cu and Zn in copper mine tailings. CHINA ENVIRONMENTAL SCIENCECE, 2014, 34(12): 3182-3188.