Abstract：An A/O biofilm reactor was developed for the treatment of petrochemical wastewater. Porous plastic spherical carriers filled with modified polyurethane foams were added in the aerobic parts to improve the degradation of organic pollutants. The A/O biofilm reactor was fed with effluent from the hydrolysis acidification tank (phase I), a mixed petrochemical and domestic wastewater with a ratio 3:1 (phase II) and petrochemical wastewater (phase III), respectively. Results showed that the removal efficiency of COD and ammonium maintained stable despite their great fluctuation in influent, indicating that the A/O biofilm reactor had a good capacity to resist the loading shock. At a hydraulic retention time of 30h, COD and ammonium removal reached 74%~77% and 96%~93%, respectively. Total nitrogen and total phosphorus removal were 58% and 79%, respectively. The performance of reactor at phase II was the best. The effluent COD and ammonium concentrations kept at (63±12)mg/L and (0.75±0.28)mg/L, respectively. Total nitrogen in the effluent existed mainly in the form of nitrate and nitrite maintained at low levels (less than 0.1mg/L), which indicated that nitrification proceeded almost completely. The molecular weight (MW) of organic matters in the influent ranged mainly in less than 1kDa (70.9%) and more than 100kDa (10.4%). In the effluent, MW of organic matters was mainly in less than 1kDa (56.6%) and 1~5kDa (26.2%). The distribution ratio of organics with a MW of higher than 10kDa decreased, indicating that A/O biofilm reactor was good at degradation of the macromolecular organics. Analysis of 454pyrosequencing showed that Proteobacteria accounted for most of sequence (60.0%), followed by Planctomycetes (16.9%) and Bacteroidetes (9.8%) at the phylum levels. At the genus levels, ammonia oxidizing bacteria (AOB) Nitrosomonas and nitrite oxidizing bacteria (NOB) Nitrospiraceae Nitrospira and denitrifier Azospira and Thermomonas were identified. NOB were highly enriched which was consistent with the good nitrification.
张楠, 初里冰, 丁鹏元, 王星, 杨琦, 王建龙. A/O生物膜法强化处理石化废水及生物膜种群结构研究[J]. 中国环境科学, 2015, 35(1): 80-86.
ZHANG Nan, CHU Li-Bing, DING Peng-Yuan, WANG Xing, YANG Qi, WANG Jian-Long. Enhanced treatment of petrochemical wastewater by using A/O biofilm reactor and analysis of biofilm community. CHINA ENVIRONMENTAL SCIENCECE, 2015, 35(1): 80-86.
