Abstract：The community diversity, abundance, and phylogeny of ammonia-oxidizing archaea (AOA) and bacteria (AOB) in the surface sediments of Beiyun River were investigated using various molecular techniques targeting the ammonia monooxygenase (amoA) genes. Diversity and abundance of bacteria were higher than those of archaea. AOB were the dominant ammonia-oxidizing microorganisms in Beiyun River. For AOB, sediment samples from main stream and tributary were cluster into two categories. In contrast, AOA communities showed relatively slight site-specific difference. Correlation analysis indicated that concentration of ammonia (NH4+) and the sum of nitrite and nitrate of sediment were the main factors affecting the community compositions of ammonia-oxidizing prokaryotes, and AOB was much more sensitive to environmental change. The amoA gene copy numbers of AOA and AOB in wet sediment ranged from 1.32×105 to 1.91×106copies/g and from 5.39×105to 8.3×106copies/g, respectively. The sediment ammonia-oxidizing prokaryotes in downstream of the dam showed the highest abundance. Phylogenetic analysis revealed that most of the cloned amoA sequences of both AOA and AOB belonged to soil/sediment group. A considerable proportion of AOB sequences were highly affiliated with soil Nitrosospira lineage. Because of the influence from wastewater treatment plant effluent, some amoA sequences were similar to those recovered from activated sludge and wastewater.
鲍林林, 陈永娟, 王晓燕. 北运河沉积物中氨氧化微生物的群落特征[J]. 中国环境科学, 2015, 35(1): 179-189.
BAO Lin-Lin, CHEN Yong-Juan, WANG Xiao-Yan. Diversity and abundance of ammonia-oxidizing prokaryotes in surface sediments in Beiyun River. CHINA ENVIRONMENTAL SCIENCECE, 2015, 35(1): 179-189.
