Abstract:To investigate the oxidative damages of diisononyl phthalate on mouse liver cells, Kunming mice were randomly grouped into five groups and orally administered with drugs daily for fourteen days; the groups included one solvent control group, four diisononyl phthalate groups. The exposure doses of diisononyl phthalate groups were 0.5, 5, 50 and 500mg/kg respectively. Some liver tissues were then made into homogenates for the measurement of ROS (reactive oxygen species), GSH (glutathione), 8-hydroxy-2-deoxyguanosine (8-OHdG) and MDA (Malondialdehyde) contents. Meanwhile, DPC (DNA-protein Crosslink) coefficients were detected from liver cell suspension. The liver contents of ROS, MDA, 8-OHdG and DPC coefficients increased gradually in a dose-dependent manner, whereas GSH content decreased accordingly. In the exposure group with the dose of 50mg/kg, ROS, GSH, 8-OHdG contents and DPC coefficients were significantly higher than those of the control group (P<0.05, P< 0.01); There were significant differences in levels of each biomarker between 500mg/kg group and control group (P< 0.05,P< 0.01). Meanwhile liver tissues were sliced for physiological observation,the results showed that tissue injury were severed gradually with the increase of exposure concentration. diisononyl phthalate at certain doses (350mg/kg) can induce oxidative stress in mice liver.
陆杰, 代园园, 罗慧, 龚金锋, 王绪杨, 武阳, 杨旭, 马萍. 邻苯二甲酸二异壬酯致小鼠肝组织氧化损伤的研究[J]. 中国环境科学, 2015, 35(1): 285-290.
LU Jie, DAI Yuan-Yuan, LUO Hui, GONG Jin-Feng, WANG Xu-Yang, WU Yang, YANG Xu, MA Ping. Oxidative damage of mouse liver tissue induced by diisononyl phthalate. CHINA ENVIRONMENTAL SCIENCECE, 2015, 35(1): 285-290.