Abstract:In order to investigate the characteristics of the catalytic activity of laccase, several types of surfactants were adopted to construct reverse micelles, using guaiacol as substrate. The activity of laccase activity was measured to determine the optimum surfactant concentration and corresponding water solubilization w0 of CTAB, Tween-80, AOT and rhamnolipid reverse micelle systems. The optimum surfactant concentrations were 20, 50, 30, 10mmol/L with corresponding water content of 20, 25, 10, 30mmol/L for CTAB, Tween-80, AOT and rhamnolipid reverse micellar systems, respectively. Under this condition the effects of surfactant type on the catalytic activity of reverse micellar systems were studied. The transforming rate of substrate in the micellar system constructed by anionic surfactant was higher than these of the others, and the rhamnolipid micelle system performed best. After 6h, the conversion rate of rhamnolipid reverse micellar systems compared with CTAB,Tween-80and AOT reverse micelle systems were 44.12%, 34.6% and 29.43% higher, respectively.