Abstract:The performance of biological nitrogen removal in treating seawater-containing wastewater is not satisfying. Here we report the acclimation of a freshwater-origin anaerobic ammonium oxidation (anammox) sludge by using seawater with increasing salt concentrations. Nitrogen removal performance, morphological characteristics and dominant strains of the bacterial community in the reactor were investigated. After 145days of acclimation, this freshwater-origin anammox sludge was fully adapted to seawater, with a total nitrogen removal rate (TNRR) reaching 2.80kgN/(m3·d). The anaerobic ammonium oxidizing rate first increased, then decreased, and then increased again when the seawater content was gradually increased from 0‰ to 30‰. A seawater content of 20‰ was the critical point during the acclimation. In terms of morphological characteristics, cell shape of the acclimated anammox bacteria was irregular and pilus-like appendages were developed on the cell wall. Change in the bacterial community composition after acclimation was also observed through DGGE.The dominant anammox strain was identified as “Candidatus Kuenenia Stuttgartiensis” based on the sequence of the 16S rDNA gene.
李智行, 张蕾, 陈晓波, 李航, 李旦. 高效耐海水型厌氧氨氧化污泥的驯化[J]. 中国环境科学, 2015, 35(3): 748-756.
LI Zhi-Xing, ZHANG Lei, CHEN Xiao-Bo, LI Hang, LI Dan. Acclimation of a highly-efficient and seawater tolerant anammox sludge. CHINA ENVIRONMENTAL SCIENCECE, 2015, 35(3): 748-756.