Abstract:A rational implementation mechanism is critical for the work efficiency and final results of a large regional environmental health investigation. It is known that the environmental health investigation is an interdisciplinary research, a complex and overlapping process. Based on understanding the essence of environmental health chain, we explored how to efficiently organize the participators and the technology strength as well as other particular implementation procedure in an environmental health risk investigation. By summarizing experiences of the national scale environmental health investigations, we proposed all the investigators participating in the regional environmental health investigation belong to four parts based on their responsibilities and role delineations and the investigation can be conducted according to a technique process with “nine steps”, which may shed light on the theory support for investigation of regional environmental health issues.