Abstract:To investigate antialgal mechanism of Solidago canadensis L. extracts, iTraq labeling technique coupled with LC-MS-MS was used to analyze differentially expressed proteins in Microcystic aeruginosa. 261 differentially expressed proteins (fold change ratio31.5) were identified, in which 220 proteins were upregulated and 41proteins were down-regulated. GO function analysis showed that the extracts of S. canadensis inhibited the algal growth by affecting metabolism process, protein catalytic activity and binding function. KEGG pathway analysis showed that top 3 enrichment pathway classes were all related to metabolism, including energy, carbohydrate and amino acid metabolism, and glycolysis/gluconeogenesis was the top enrichment pathway. This study elucidated the molecular targets of the extracts of S. canadensis, thereby giving insight into the antialgal effects at the molecular level and providing a new way to study the antialgal mechanism of plant allelopathic compounds.
黄莹莹, 白羽, 王艳, 孔海南. 基于iTraq技术的加拿大一枝黄花提取物作用下铜绿微囊藻细胞差异表达蛋白[J]. 中国环境科学, 2015, 35(6): 1822-1830.
HUANG Ying-Ying, BAI Yu, WANG Yan, KONG Hai-南. Differentially expressed proteins in Microcystic aeruginosa with Solidago canadensis L. extracts using iTraq labeling technique. CHINA ENVIRONMENTAL SCIENCECE, 2015, 35(6): 1822-1830.