Abstract：A high performance liquid chromatographic (HPLC) method was developed for the determination of phthalic acid (PA) in urine after alkaline hydrolysis of the phthalate metabolites to PA. The content of the total PA in male adult SD rat urine was measured after separated or combined exposure to dibutyl phthalate (DBP) and di 2-ethyl hexyl phthalate (DEHP), and then the relationships between exposed PAEs molar quantities and the molar concentration of the urine PA were analyzed by linear regression. The results showed that the total amount of urine PA increased as the exposure increased. The mean percentage of PAEs intake excreted in the urine was found to be 65.04% for DBP exposure alone and 55.00% for DEHP exposure alone and 38.35% for combined exposure to the two. The contents of urinary PA were well correlated with PAEs exposure levels with the correlation coefficient being 0.92 for DBP(P<0.01), 0.56 for DEHP (P<0.01) and 0.93 for combined DPB and DEHP (P<0.01). The findings suggested that PA in urine after alkaline hydrolysis of the phthalate metabolites could evaluate the total exposure level of PAEs, which may be a valid exposure biomarker of PAEs used in risk surveillance of human exposure to PAEs in future.
柳春红, 张明明, 赖玉婷, 褚玥, 李健俊, 方敏婷. 邻苯二甲酸酯暴露的大鼠尿液生物标志物研究[J]. 中国环境科学, 2015, 35(9): 2868-2873.
LIU Chun-Hong, ZHANG Ming-Ming, LAI Yu-Ting, CHU Yue, LI Jian-Jun, FANG Min-Ting. Urine biomarker of exposure to phthalate in rat. CHINA ENVIRONMENTAL SCIENCECE, 2015, 35(9): 2868-2873.